An emerging counter to plant pathogens is biocontrol through antibiosis. This project aimed to discern how antibiotic production by Pseudomonas fluorescens PF-5 was affected in the presence of fungal metabolites extracted from two unknown fungi (F-12 and F-42). Antibiotics studied included 2,4-diacetylphloroglucinol, monoacetylphloroglucinol, pyoluteorin and pyrrolnitrin. Antibiotic production was shown to be significantly affected by the fungal extracts, but this significance could be due to the solvent with which the extracts were acquired. Additional research is needed to determine how the solvent is affecting the molecular pathway of interactions between the fungal metabolites and antibiotics, or the experimental method could be further studied and possibly changed to employ a different solvent.
Brittany Barnwell, ’13
Major: Biochemistry and Molecular Biology
Sponsor: Brian Nowak-Thompson