2,5-Dialkylresorcinol (DAR) is a compound with both antifungal and antibiotic properties, and has the potential to be used in the biological control of plant pathogens. The gene cluster encoding for DAR has been identified in Pseudomonas chlororaphis strains 30-84 and O6, but these strains have not previously been shown to produce DAR. I was successful in creating a DAR standard by purifying extract from Ps. fluorescens BL915 grown in Luria-Bertani media. The culture extract was purified using flash-silica chromatography, and the isolated sample was confirmed to be DAR using H-NMR, 13C-NMR, and HPLC. Ps. chlororaphis strains 30-84 and O6 were grown in various media and the culture extracts were scanned for DAR using TLC. Initial results, including HPLC evidence, show that Ps. chlororaphis 30-84 produces DAR when grown in Kings Media B. I was unsuccessful in identifying a media in which Ps. chororaphis O6 produces DAR.
Lauren Morris, ’12
Majors: Mathematics and Statistics, Biochemistry and Molecular Biology
Sponsor: Brian Nowak-Thompson