This research focused on the role of Toll-like Receptor 2 (TLR 2) in the development of adipose inflammation in obesity. Both Toll-like Receptor 4 and Toll-like Receptor 2 have been shown to
be critical components of the innate immune response to bacterial pathogens. Moreover, TLR 4 has been shown to bind saturated fats directly and develop an inflammatory response. Knowing the similarities in function between TLR 2 and 4, TLR 2 was hypothesized to be required for the production of inflammation in adipose tissue in response to a diet high in saturated fat. In a similar fashion, RNA of several inflammatory cytokines was measured quantitatively using realtime-PCR once cDNA templates were synthesized. Tissue was harvested from wild type and TLR-2 -/- mice, which were fed either a control or a high fat diet. Although inflammation was not completely absent in the TLR-2 -/- mice, results showed the receptor has a role in regulating tumor necrosis factor and Interleukin 10 in acute obesity in response to a diet high in saturated fat.
Nate Olafsen, ’08 Monona, IA
Major: Biochemistry and Molecular Biology
Sponsor: Barbara Christie-Pope