We investigated the effects of a short term high fat (21% milk fat), high cholesterol (0.15%) diet in a murine obesity model. Our mice were fed the high fat diet for 10 weeks (~8-10 years in a human), a standard chow diet for 10 weeks, or the high fat diet for 5 weeks followed by 5 weeks of the standard chow diet. We looked at chemokines indicative of inflammation, macrophage infiltration, total cholesterol levels, and soluble ICAM-1 levels. The 6 chemokines that were investigated were all significantly increased in the 10 week high fat mice while they were not in the 5 on 5 off mice. Macrophage infiltration was significantly increased in the 10 week high fat mice while again the 5 on 5 off mice were at control levels. sICAM-1 and cholesterol levels were also significantly higher in the 10 week high fat mice and at control levels in 5 on 5. This shows that the effects of short term obesity on the inflammatory response in adipose tissue can be reversed by returning to a healthy diet. This means that the adverse health effects related to inflammation in obesity may be reversed by diet and exercise.
Tyler Ulland, ’06 Austin, MN
Major: Biochemistry and Molecular Biology
Sponsor: Barbara Christie-Pope