Large Tumor Suppressor Kinase 1 (LATS1) is a serine/threonine kinase previously associated with regulation of cellular proliferation and enhancement of apoptosis. However, the specific protein-protein interactions of LATS1, particularly in the brain, are not entirely known. In order to elucidate this interaction, the objective of the presented project centered on screening novel neural interacting partners of LATS1. Potential LATS1 interacting partners were identified through the use of a yeast-two-hybrid screen between LATS1 and a rat brain-derived cDNA library. Potential interacting partners were subsequently assessed for co-immunoprecipitation with LATS1 when co-expressed in human embryonic kidney cells (HEKC). The results of this study confirmed a previously found interaction between LATS1 and proteins AMOT and NEDD-4 and suggested a potential novel interaction between LATS1 and proteins Rab 11 FIP2 and KIBRA.
Ariadne Penalva, ’15
Bahía de Banderas, Mexico
Biochemistry & Molecular Biology
Sponsor: Craig Tepper