We used organic synthesis to place a deuterium atom at the 2″ position of the deoxyribose sugar of adenosine. After the synthesis, proton nuclear magnetic resonance (1H NMR) was used to determine if the deuterium labeling was successful. In a separate synthesis, we deuterium-labeled a deoxyribose ring that contained no base (abasic) at the 1′/1″ position. The labeled adenosine will be incorporated into a DNA dodecamer to provide a standard against which a dodecamer containing a damaged nucleotide, in this case the labeled abasic sugar, will be compared. In the future, 1H NMR will be performed on the abasic species to determine if the labeling was successful.
Brianna Medrano, ’09 Marshall, MO
Majors: Chemistry-ACS Certified, Biochemistry and Molecular Biology
Missouri State University
Sponsor: Craig Teague