Denaturing gradient gel electrophoresis (DGGE) is a technique that has been used to estimate the diversity in bacterial samples based on the melting behaviors of DNA segments. DGGE may provide a new, more efficient manner to identify unknown bacteria, as current processes to identify unknown bacteria are time consuming and costly. The unknown bacterial PCR products of similar molecular weight but different base pair sequences are separated by the denaturing gradient. Different bacterial strains appear to form unique banding patterns when the DNA is amplified with multiple primer sets; this may eventually allow unknown bacteria to be identified solely by DGGE and comparison to a database.
Garrett Feddersen, ’08 Ida Grove, IA
Majors: Biochemistry and Molecular Biology, Politics
Jeffrey Cardon, Ph.D.
Sponsor: Jeffrey Cardon