A sensitive, analytically simple method with high-throughput capability is being developed for the quantitative analysis of N-acetylcysteine (NAC) in human plasma. Standard concentrations of NAC were dissolved in phosphate buffer, processed with sodium nitrite, and separated using reverse-phase high performance liquid chromatography (HPLC); detection and quantification were accomplished by UV Vis spectroscopy at 333nm. Characteristic peaks for NAC and mercapto-propionyl-glycine (MPG), the internal standard, were achieved with adequate separation and improved retention times from previously reported methods.
Jeffrey Welder, ’07 West Des Moines, IA
Majors: Biochemistry and Molecular Biology, Philosophy
Sponsor: Craig Tepper